產(chǎn)品英文名稱：CHO ELISA kit
產(chǎn)品中文名稱：CHO 細(xì)胞 ELISA 試劑盒

CHO ELISA kit 的應(yīng)用
本試劑盒是用于確定在CHO宿主細(xì)胞重組表達(dá)產(chǎn)品 中CHO蛋白污染存在。該試劑盒只用于研究和制造使用，不適合于人類或動(dòng)物的診斷應(yīng)用。

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CHO細(xì)胞 ELISA 試劑盒的英文描述
The antibodies used in the kit were generated against and affinity purified from a cell lysate of CHO cells grown in serum supplemented media. All three of the Cygnus CHO HCP kits recognize a qualitatively vast majority of the same HCPs. The potential exists that the quantitative value reported for a given sample type may differ between the kits, therefore we cannot predict which kit will be most suitable for your applications. Formal qualification must be performed on each different product, to determine which of the 3 kits are superior. For labs looking at CHO HCP contamination for the first time, we suggest evaluating our CHO HCP ELISA, 3G, Catalog # F550. If this method proves inadequate then we suggest evaluating CHO HCP kits, Catalog #CM015 and F015.

CHO ELISA kit 的使用步驟規(guī)則
The CHO Host Cell Protein assay is a two-site immunoenzymetric assay. Samples which may contain CHO proteins are reacted with an affinity purified alkaline phosphatase labeled antibody. This reaction either takes place in a test tube in a sequential assay mode or simultaneously in microtiter wells coated with an anti-CHO HCP capture antibody.The two-step sequential protocol or the one step simultaneous
protocol result in the formation of a sandwich complex of solid phase (capture) antibody-CHO protein-enzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate p-nitrophenyl phosphate (PNPP) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of CHO proteins present.

CHO ELISA kit 的保存和穩(wěn)定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.
* Reconstituted wash solution is stable until the expiration date of the kit.

CHO ELISA kit 的使用注意事項(xiàng)
*只用于研究或制造。
*在用本試劑盒的試劑濃度，其他沒(méi)有被認(rèn)為是有害的。
*本試劑盒只能由合格的技術(shù)人員使用。

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Pall   15950-017  Ultroser Serum Substitute 血清替代品 20mL/瓶

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BD 762165   PAXgene Blood RNA Tube RNA樣本收集管 100管/盒

LONZA 50150   SeaKem® Gold Agarose 金瓊脂糖粉 125g/瓶

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Nunc 159910   175cm2易用培養(yǎng)瓶,PS,已滅菌,瓶蓋:過(guò)濾蓋 30個(gè)/箱

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Corning   431080    175cm⒉細(xì)胞培養(yǎng)瓶，Angled頸，透氣蓋 50個(gè)/箱

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Corning   3290     75cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 100個(gè)/箱

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BD-Falcon   352360   Cell Strainer, 100µm, Yellow 100um細(xì)胞濾網(wǎng) 50個(gè)/箱

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BD Falcon 352096 15mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱

BD BioCoat 354234   Matrigel Basement Membrane Matrix(標(biāo)準(zhǔn)型) 10mL/瓶

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Nalgene  5100-0001 Cryo 1℃ Freezing Container梯度降溫凍存盒 1個(gè)/箱

Nalgene    5026-0909   CryoBoxes 凍存盒 9X9 陣列，管瓶1.2/2.0mL   24個(gè)/箱

Nalgene   2250-0130 50L 可高溫高壓操作的細(xì)口大瓶（帶手柄）   1個(gè)/箱

Nalgene   2319-0050    20L 可高溫高壓滅菌的細(xì)口大瓶（帶放水口）    1個(gè)/箱

Hyclone  SH30084.03 FBS 優(yōu)等澳洲胎牛血清 500mL/瓶

PeproTech   100-18B-500   Recombinant Human FGF-basic (154 a.a.)因子 500ug/支

Parafilm PM-996   Parafilm® M Laboratory Film 封口膜 12卷/箱